Coding

Part:BBa_K3990015:Design

Designed by: Li Wenxi   Group: iGEM21_SMS_Shenzhen   (2021-10-01)


RoxB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1937
    Illegal BamHI site found at 186
    Illegal BamHI site found at 1386
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1078
    Illegal NgoMIV site found at 1429
    Illegal NgoMIV site found at 1465
    Illegal AgeI site found at 598
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Rubber cleavage enzyme, the rubber oxygenase RoxBXsp (RoxB of Xanthomonas sp. strain 35Y)


Source

From Xanthomonas sp. strain 35Y.

References